| 细胞系鉴定 |
| 应用于生物医学研究领域的哺乳动物细胞被错误鉴定和交叉污染的问题，一直是一个普遍存在的突出问题。据统计，国外实验室有20%左右的细胞株被错误鉴定和交叉污染，NIH和ATCC近两年都对此发出呼吁，要求研究者对细胞进行鉴定。近年来，大量研究表明STR基因分型方法是进行细胞交叉污染和性质鉴定的最有效和准确的方法之一，STR基因分型应用于细胞鉴定已被ATCC等机构强烈推荐。作为ATCC标准委员会成员，在国内率先推出了细胞STR鉴定服务并与多家研究机构、细胞中心、生产厂商等建立长期合作关系。 |
| 高度丰富信息：同时分析多个STR基因座，可以准确的判断交叉污染和细胞类型。 |
| 高度灵敏：可以检测最低0.1ng DNA（约15个二倍体基因组），微量样本即可满足需求，并且可以检测到早期污染。 |
| 高通量和系统化平台：采用自动化分析系统可大量检测及数据分析，结果更加准确、客观。 |
| 人类细胞20个基因座检测：(1200元每样) |
| 是目前基因座最多的检测服务，兼容以上各组数据之外更添加4个高度多态性位点——D19S433、D6S1043、D12S391和D2S1338，具有更高的准确性。 |
| 人鼠细胞交叉污染检测 |
| 国内首创技术，采用人鼠复合扩增体系检测人类细胞中的小鼠细胞污染，主要应用在干细胞研究领域。同时可以用于判断人鼠细胞混淆的情况。 |
（1）高度丰富信息，同时分析超过15个STR基因座，可以准确判断交叉污染和细胞类型；
（2）高灵敏度，可以检测最低至0.1ng DNA(约15个二倍体基因组)，微量污染也可检测；
（3）高通量和系统化平台，日处理量上千个检测，数据分析比对采用自动化软件分析系统。 |
| 适用范围： |
| 将人源细胞系用于研究和生产的用户，包括但不限于以下领域：医学，遗传学，药物开发，疫苗研制，生物技术和制药行业，再生医学，基础科学，HIV的检测和治疗以及细胞生物学。 |
| 各大细胞保藏库 ?科技期刊的同等评审 ?学术团体 ?政府部门和私营机构 |
| 送样要求： |
| 细胞悬液（细胞数≥106 个）或基因组DNA（≥20μl，浓度≥50ng/μl）。 |
| 提供结果： |
| STR分型的图谱和检测结果的书面报告。 |
| 本中心为国内外客户提供专业的细胞系鉴定:细胞身份鉴定，ATTC人源细胞系鉴定, 干细胞和肿瘤细胞鉴定等服务 |
| 实验室联系方式:021-56096592 Email:yonghuigu@hotmail.com或Email: strdna@163.com |
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| Human Cell STR Genotyping Service (Identification of Cell Line) |
| Cell contamination and identification errors are very common phenomenon nowadays. This will probably impose extremely big influence on the subsequent research work, especially in the field of the study on stem cell and tumor cell. |
| According to statistics, almost 15%-25% of the research theses were misled to the wrong conclusions because of the great many wrongly identified and cross contaminated cells. Thus, it is quite important to supervise and control the contamination and mutation occurred in cells. |
| Cell Strain STR Genotyping Service is a fast, economic and accurate method of identifying the contaminated and wrong cells. It has become the conventional means of cell identification in the international society. Such STR genotyping data of each strain of cells have also been contained in the ATTC. |
| Characteristics: |
1.Abundant information makes it possible to analyze over 15 pieces of STR locus at the same time, and to accurately judge the cross contamination and the cell type.
2.Due to the high sensitivity, it could detect 0.1ng DNA(approximately equal to 15 diploid genome), and even the trace contamination.
3.Its High-throughput and systematic platform enables to handle thousands of detection within one day. All the works of analyzing and comparing the data are done through automated software analysis system. |
| Applicable fields: |
1.The customers who apply the human cell line on the research and production, including but not limited in the following fields: medical science, genetics, drug development, vaccine development, biotechnology and pharmaceutical industry, regenerative medicine, basic science, detection and treatment of HIV, and cell biology;
2.major cell treasures libraries;
3.the same assessments of scientific and technical journals;
4.academic organizations;
5.government departments and the private sectors; |
| Our company provides foreign and domestic customers with the professional cell line identification services: cell identification, ATTC human cell line identification, and stem cell and tumor cell identification, etc. |
| Laboratory contact: |
| Ms. Gu |
| Mobile: +86 15601691258 |
| Email: yonghuigu@hotmail.com; or strdna@163.com |
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